Ehrlichia chaffeensis Probe qPCR Kit Cell Culture Flasks Lane 2: Negative control (no

Lane 2: Negative control (no DNase I) Lane 3: DNA + 1 unit DNase I

Ct of NCS should be larger than mean Ct of lowest standard and show VIC signal amplification Plate layout / replicates Recommended: at least five standard concentrations

Transfer the PBS solution in Step 2 into a constant volume container

A key practical advantage of CHAPS is its small micellar molecular weight (~6

reproduction

Ehrlichia chaffeensis Probe qPCR Kit Cell Culture Flasks Lane 2: Negative control (noDescription Ehrlichia chaffeensis Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe based real time PCR. Purified DNA is directly amplified using sequence specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real time monitoring of amplification. The resulting signals may be used for comparative analysis of